Search results for “assay”

About 82 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching assay — open any to read the full text, or download the PDF or XML.

Identification of Antioxidant Activity of Medicinal Plant Extracts Through a Modified and Rapid ABTS Colorimetric Assay

Nov 2026 DOI 10.14302/issn.2471-2140.jaa-26-6462
Anthony L. NewsomeCorresponding author

The consumption of antioxidant rich plants is a common human activity. Therefore, easily used, time efficient and effective antioxidant assays of plants consumed by humans are of increasing importance. The ABTS [2,2’-azinobis-(3-ethtlbenzothiazoline-6-sulfonic acid)] cation radical is commonly used…

Comparative Analysis of Five Commercial RT-PCR Diagnostic Assay for Detection of Covid-19

Sep 2023 DOI 10.14302/issn.2692-1537.ijcv-23-4660
Saeid Amel JamehdarCorresponding author

SARS-CoV-2 real-time reverse-transcription PCR (rRT-PCR) is the most effective testing system available to combat COVID-19, given the absence of any specific treatment or vaccine. Moreover, numerous SARS-CoV-2 rRT-PCR kits have been approved under emergency-use-authorization (EUA) worldwide. In this…

The Evolution of the Enzyme Immunoassay/Enzyme-Linked Immunosorbent Assay

Aug 2021 DOI 10.14302/issn.2326-0793.jpgr-21-3917
Leonid TarassishinCorresponding author

50 years ago the Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay, mostly known as ELISA was developed. This is a powerful but simple method that is very widely used in the diagnostic practice, as well as in biomedical research. During this time a number of ELISA modification were developed that…

The Feasibility of Enzyme Immunoassay Tests in the Absence of a Conventional Source of Electricity

Oct 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3517
Camille S.U. DOSSOUCorresponding author

Background of the Study African  countries are facing frequent blackout. Thus in sub Saharan region, due to frequent power cut, the laboratory professionals find sometimes difficulty to carry out earlier diverse diagnostic tests. Objective The  aim of this work is to evaluate the feasibility of enzy…

Use of Microfluidic Assays to Develop Reliable and Economic Nucleic Acid Application Technologies, Employing MicroRNAs for the Diagnostic Screening of Colon Cancer in Human Stool in Low-Resource Settings

Oct 2020 DOI 10.14302/issn.2379-7835.ijn-20-3418
Farid E. AhmedCorresponding author

Isolation methods that employ readily-available inexpensive supplies on the open market, which are reliable, as well as economical, such as nucleic acid amplification techniques (NAAT) based on microfluidic technology in low-resource research settings (LRRS) that meets the ASSURED guidelines are ess…

Use of Microfluidic Assays to Develop Reliable and Economic Nucleic Acid Application Technologies, Employing MicroRNAs for the Diagnostic Screening of Colon Cancer in Human Stool in Low-Resource Settings

Jun 2020 DOI 10.14302/issn.2379-7835.ijn-19-3123
Farid E. AhmedCorresponding author

Isolation methods that employ readily-available inexpensive supplies on the open market, which are reliable, as well as economical, such as nucleic acid amplification techniques (NAAT) based on microfluidic technology in low-resource research settings (LRRS) that meets the ASSURED guidelines are ess…

The Current Immunoassays and Emerging Immunogenomic Approaches for Immunomonitoring Cancer and Infectious Diseases

Jan 2019 DOI 10.14302/issn.2572-3030.jcgb-18-2527
Xi ZhangCorresponding author

As remarkable advances have been made in immunotherapies, the overall goal of immunotherapy has become the selection of patients and evaluating the benefits of treatment. One of the major obstacles to develop immunotherapies is the lack of effective immune monitoring. Monitoring of key changes in th…

The Development and Evaluation of A Multiplex Real-Time PCR Assay for the Detection of ESBL Genes in Urinary Tract Infections

Aug 2018 DOI 10.14302/issn.2690-4721.ijcm-18-2217
Shivanthi SamarasCorresponding author

Background Overuse of beta-lactam antibiotics has lead to selection for extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae, a major cause of antibiotic resistant urinary tract infections (UTIs). Standard detection methods are time-consuming, with disputed accuracy. This study describe…

Polysaccharide Transglycosylases: A Survey of Assay Methods

May 2018 DOI 10.14302/issn.2832-5311.jpcd-18-2077
Vladimír FarkašCorresponding author

Polysaccharide transglycosylases (PTGs) are a unique group of glycoside hydrolases playing important roles in the formation and modification of plant and fungal cell walls. Their action involves cutting the molecule of the polysaccharide substrate at the glycosidic bond, followed by transfer of the…

Calcium Transient Assays for Compound Screening with Human iPSC-derived Cardiomyocytes: Evaluating New Tools

Jan 2017 DOI 10.14302/issn.2574-4372.jesr-16-1395
Tetsuro WakatsukiCorresponding author

Calcium (Ca2+) plays a central role in regulating many biological processes in the cell from muscle contraction to neurotransmitter release. The need for reliable fluorescent calcium indicator dyes is of vast importance for studying many aspects of cell biology as well as screening compounds using p…

Correlation of Cryptococcal Antigen Assay with C-reactive Protein as Serum and Urine Biomarker in Cryptococcal Meningitis: Experience in a Tertiary Hospital

Jul 2013 DOI 10.14302/issn.2324-7339.jcrhap-12-150
Bineeta KashyapCorresponding author

Aims and Objectives: The incidence of cryptococcal meningitis caused by Cryptococcus neoformans has risen markedly over the past 20 years as a result of theHIV epidemic and increasing use of immunosuppressive therapies. The objectives of this study were to isolate and identify Cryptococcus neoforman…

The Advantages of Employing the Immune Response Mediated by Immunoglobulins in Preclinical and Clinical Research: An Essential Review

Sep 2026
Yilkal Tariku BelayCorresponding author

Immunoglobulins, commonly known as antibodies, are glycoprotein molecules present on B lymphocytes and within the bloodstream. They are essential components of the immune response, as they detect the harmful molecules of test compounds and stimulate the immune system to manifest proportional counter…

Exploring the Mechanism of Complex Lemon-Angelica Sinensis-Boswellia Essential Oil on Anxiety Disorders with Melasma Through Network Pharmacology and Experimental Validation

Dec 2025
Liping LiuCorresponding author

The incidence rate of melasma is notably high among patients with anxiety disorders. Aromatherapy primarily influences the physiological and psychological states of individuals through the inhalation or application of essential oils, thereby facilitating the treatment or alleviation of various condi…

Diagnostic Performance of Smear Microscopy and Xpert MTB/RIF Versus MGIT Culture in Republic of Congo

Nov 2025 DOI 10.14302/issn.2690-4721.ijcm-25-5786
Darrel Ornelle Elion AssianaCorresponding author

In the Republic of the Congo, tuberculosis (TB) remains a major public health concern. Although the GeneXpert MTB/RIF assay is the WHO-recommended first-line diagnostic test, smear microscopy is still used for treatment monitoring and in facilities where molecular testing is limited. Evaluating the…

Fecal Shedding, Antimicrobial Resistance and In Vitro Biofilm formation on Simulated Gallstones by Salmonella Typhi Isolated from Typhoid Cases and Asymptomatic Carriers in Nairobi, Kenya

Apr 2024 DOI 10.14302/issn.2690-4721.ijcm-24-5030
John S. GunnCorresponding author

Typhoid fever, caused by the human restricted pathogen Salmonella Typhi, remains a major global public health concern. Even after successful treatment, approximately 3-5% of patients with typhoid fail to clear the bacteria within one year and become chronic carriers. Most typhoid carriers have galls…

The Application of Immunoglobulins Immune Response in the Discovery and Development of Safe Therapeutic Agents: A Review Article

Jan 2024 DOI 10.14302/issn.2328-0182.japst-23-4771
Yilkal Tariku BelayCorresponding author

Background Immunoglobulins are bio-receptors found embedded in the cell membrane with a biological role that detects the harmful molecules of a test compound. These bio-receptors interface between a biological system and its external environment that transduce information to the effector via interme…

Journal of Enzymes Open Access

Enzyme Immobilization on Polypropylene Film: A Role Model for Biocatalytic Polymer Membranes?

Dec 2023 DOI 10.14302/issn.2690-4829.jen-23-4799
Patrizia GartnerCorresponding author

Polymer electrolyte membrane (PEM) technologies hold promise for sustainable energy solutions, yet pinhole-related challenges persist. Our research introduces a novel biohybrid approach to self-healing, enhancing multiple healing cycles with minimal membrane disruption. Initial steps involve immobil…

A Review on Monkey Pox: Role of One Health Approach Against Monkey Pox

Oct 2023 DOI 10.14302/issn.2641-4538.jphi-23-4622
Tariku Geinoro AlleyoCorresponding author

The Ortho-poxvirus virus, which causes monkey pox, is a member of the Poxviridae genus. It was initially found in primates. In 1970, the Democratic Republic of the Congo reported the first instance of monkey pox. From there, it spread to a number of countries both inside and outside of Africa. There…

Camel Brucellosis in Ethiopia: Seroprevalence and Associated Risk Factor

Oct 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4532
Sultan Abda NejaCorresponding author

Camels are a significant source of income for nomadic populations in many developing countries, including Ethiopia. Camels are well adapted to dry and semi-dry regions, providing income, food security, and transportation. However, camel production and productivity are constrained by infectious disea…

A Review of Attempts to Identification and Antifungal Susceptibility of Dermatophytes (Microsporum Canis and Tricophyton Mentagrophytes) Isolated from Infected Cats and Dogs with Experimental Dermatophytosis of Guinea Pigs

Jul 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4510
Sohir YoussefCorresponding author

Dermatophytosis affect companion animal’s skin and keratin appendages as cats and dogs, resulting in red, scaly, itchy, bald, and raised patches like ring. The three main groups are Microsporum, Trichophyton and Epidermophyton. This study collected samples of skin scrapping and hairs from 130 cats a…

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